Laboratory Technician Grade II Questions and Answers

 PROVISIONAL ANSWER KEY
Question Paper Code: 174/2023/OL
Category Code: 103/2023
Exam: Laboratory Technician Grade II (SR for ST)
Date of Test 28-12-2023
Department Health Services

Question1:-Normal reticulocyte count
        A:-50-100 x 10⁹ /L
        B:-4-11 x 10¹² /L
        C:-3-5 x 10¹² /L
        D:-6-15 x 10⁹/L
        Correct Answer:- Option-A

Question2:-The coagulation factor not involved in intrinsic pathway of blood coagulation
        A:-Factor IX
        B:-Factor VII
        C:-Factor VIII
        D:-Factor XII
        Correct Answer:- Option-B

Question3:-Basophilic stippling occurs in
        A:-Thalassemia
        B:-Megaloblastic anaemia
        C:-Lead poisoning
        D:-All of the above
        Correct Answer:- Option-D

Question4:-Reducing agent used in sickling test
        A:-Potassium sulphate
        B:-Sodium metabisulphite
        C:-Sodium nitropreusside
        D:-Sodium Hypochlorite
        Correct Answer:- Option-B

Question5:-Hyper segmented neutrophils are found in
        A:-Iron deficiency anaemia
        B:-Acute lymphoblastic leukemia
        C:-Macrocytic anaemia
        D:-Acute myecogenous leukemia
        Correct Answer:- Option-C

Question6:-Which of the following are used as prostatic marker
        A:-Acid phosphatase
        B:-Citric acid
        C:-Zinc
        D:-All of the above
        Correct Answer:- Option-D

Question7:-Reagent used for detection of ketone bodies by dipstick method
        A:-Sodium nitroferricyanide
        B:-Ferric chloride
        C:-Potassium iodide
        D:-Ortho-toluidine
        Correct Answer:- Option-A

Question8:-Which of the following organisms are destroyed when CSF samples are refrigerated
        A:-S. Pneumoniae
        B:-N. Meningitidis
        C:-E.Coli
        D:-Salmonella typhi
        Correct Answer:- Option-B

Question9:-Black tarry stool are indicative of
        A:-Obstructive jaundice
        B:-Cholera
        C:-Gastrointestinal haemorrhage
        D:-Food poisoning
        Correct Answer:- Option-C

Question10:-Foul smelling sputum is observed in
        A:-Bronchiectasis
        B:-Pulmonary oedema
        C:-Asthma
        D:-Pneumonia
        Correct Answer:- Option-A

Question11:-Cryoprecipitate is administered to patients suffering from
i.   Haemophilia
ii. Thrombocytopenia
iii. Leucopenia
iv. Von Willebrand disease
        A:-i and ii
        B:-ii and iii
        C:-i and iv
        D:-ii and iv
        Correct Answer:- Option-C

Question12:-Membrane stabilizer used in SAG-M blood bags
        A:-Mannitol
        B:-Dextrose
        C:-Adenine
        D:-Citric Acid
        Correct Answer:- Option-A

Question13:-Which one of the following immunoglobulin crosses the placenta frequently
        A:-IgM
        B:-IgA
        C:-IgE
        D:-IgG
        Correct Answer:- Option-D

Question14:-Which of the following statement is correct for Bombay blood group
        A:-persons with Bombay blood group carries HAg
        B:-Bombay blood group person cannot receive 'O' blood group
        C:-Bombay blood group in detected using forward grouping
        D:-Bombay blood group persons lack anti H antibodies
        Correct Answer:- Option-B

Question15:-Anti AI lectins are extracted from
        A:-Ulex europeus
        B:-Vicia graminea
        C:-Dolichos biflorus
        D:-Arachis hypogea
        Correct Answer:- Option-C

Question16:-Microtome which is ideal for cutting sections of very large blocks of tissue
        A:-Sliding microtome
        B:-Sledge microtome
        C:-Rocking microtome
        D:-Rotary microtome
        Correct Answer:- Option-B

Question17:-All are mercury containing fixatives except
        A:-Heidenhain susa
        B:-Zenker's fluid
        C:-Helly's fluid
        D:-Gander's fluid
        Correct Answer:- Option-D

Question18:-Best mounting media for fat stains
        A:-Apathy medium
        B:-DPX
        C:-Glycerine jelly
        D:-Canada Balsum
        Correct Answer:- Option-C

Question19:-All are true about frozen section except
        A:-Structural details may become distorted
        B:-Serial sections can be obtained
        C:-Polychrome methylene blue can be used for staining
        D:-Adhesive not required for unfixed tissue
        Correct Answer:- Option-B

Question20:-Following are special stains for hemosiderin except
        A:-Tirmann - Schmelzer's turnbull blue technique
        B:-Perl's prussian blue
        C:-Glenner's method
        D:-Stein's method
        Correct Answer:- Option-D

Question21:-All are true about sex chromatin except
        A:-Buccal mucosal scrapings from inside the cheek is obtained for sex chromatin study
        B:-The most reliable staining is hematoxylin and Eosin
        C:-Fixative used is equal parts of ether and alcohol
        D:-Sex chromatin is seen as an intensely staining dot lying against the nuclear membrane
        Correct Answer:- Option-B

Question22:-All are nuclear fixatives except
        A:-New comer's fluid
        B:-Clarke's fluid
        C:-Carnoy's fluid
        D:-Champy's fluid
        Correct Answer:- Option-D

Question23:-All are true about Shorr's staining except
        A:-Used for hormonal assessment
        B:-Not very useful in detecting malignancies
        C:-Nuclear definition and cytoplasmic transparency is better than pap stain
        D:-Contain biebrich scarlet, orange G and fast green
        Correct Answer:- Option-C

Question24:-"NAF" solution in cell block technique contain
        A:-Formaldehyde
        B:-Glycerin
        C:-Ethanol
        D:-Eosin
        Correct Answer:- Option-B

Question25:-All are advantages of liquid based cytology except
        A:-Number of slides per patient are reduced
        B:-Improves the background of microscopy
        C:-Improves cell preservation
        D:-Cause drying of cells during preparation
        Correct Answer:- Option-D

Question26:-In Karyotyping, shortest acrocentric chromosomes are seen in which group?
        A:-B group
        B:-D group
        C:-E group
        D:-G group
        Correct Answer:- Option-D

Question27:-V chromosome is a _________ chromosome
        A:-Metacentric
        B:-Submetacentric
        C:-Centric
        D:-Acrocentric
        Correct Answer:- Option-D

Question28:-Which banding technique employs fluorescence for chromosomal studies
        A:-G banding
        B:-Q banding
        C:-C banding
        D:-H banding
        Correct Answer:- Option-B

Question29:-Turner syndrome is
        A:-XXY
        B:-48 XXXY
        C:-X0
        D:-49 XXXYY
        Correct Answer:- Option-C

Question30:-Most commonly employed mitogen for chromosome preparation from whole blood culture
        A:-Phyto hemagglutinin
        B:-Lipo polysaccharide
        C:-Concanavalin A
        D:-Pokeweed mitogen
        Correct Answer:- Option-A

Question31:-Which of the following is a High level disinfectant?
        A:-70% Ethyl alcohol
        B:-Hydrogen peroxide
        C:-Quaternary ammonium compound
        D:-Phenol
        Correct Answer:- Option-B

Question32:-Who is known as the 'Father of Antiseptic surgery'?
        A:-Joseph Lister
        B:-Louis Pasteur
        C:-Edward Jenner
        D:-Robert Koch
        Correct Answer:- Option-A

Question33:-Polymerase Chain Reaction (PCR) was developed by
        A:-Ernst Ruska
        B:-Rosalyn Yallow
        C:-Kary B Mullis
        D:-Karl Landsteiner
        Correct Answer:- Option-C

Question34:-Which of the following is not a feature of Electron Microscope?
        A:-Viruses can be detected
        B:-The object appears bright against a dark background
        C:-Intra cellular structures of bacteria can be seen
        D:-Electromagnet lens is used
        Correct Answer:- Option-B

Question35:-Infectious plastic wastes should be segregated in which colour bag?
        A:-White
        B:-Yellow
        C:-Blue
        D:-Red
        Correct Answer:- Option-D

Question36:-Which of the following is an anaerobic medium
        A:-Thioglycollate broth
        B:-Selenite F broth
        C:-Bile broth
        D:-Tetrathionate broth
        Correct Answer:- Option-A

Question37:-Multiple ring forms are the characteristic feature of
        A:-P.vivax
        B:-P.falciparum
        C:-P.Ovale
        D:-P.malariae
        Correct Answer:- Option-B

Question38:-Which of the following has no cystic stage?
        A:-Trichomonas vaginalis
        B:-Giardia lamblia
        C:-Enteromonas hominis
        D:-Dientamoeba fragilis
        Correct Answer:- Option-A

Question39:-An AIDS patient came to the out patient dapartment complaining loos stools. On acid fast staining, stool showed 4-6μm size oocyst. The most probable
diagnosis is
        A:-Cryptococcus
        B:-Cyclospora
        C:-Cystoisospora
        D:-Cryptosporidium
        Correct Answer:- Option-D

Question40:-Which of the following is NOT a nematode with small intenstine as habitat
        A:-Ascaris lumbricoides
        B:-Ancylostoma duodenale
        C:-Enterobius vermicularis
        D:-Necator americanus
        Correct Answer:- Option-C

Question41:-Larva currens is caused by
        A:-Toxocara canis
        B:-Toxocara cati
        C:-Ascaris lumbricoides
        D:-Strongyloides stercoralis
        Correct Answer:- Option-D

Question42:-Which of the following DO NOT float in saturated salt flotation technique of stool
        A:-Fertilised egg of round worm
        B:-Unfertilised egg of round worm
        C:-Ovum of pinworm
        D:-Ovum of hook worm
        Correct Answer:- Option-B

Question43:-Diarrheal stool with rice water appearance is a characteristic feature of the disease
        A:-typhoid
        B:-bacillary dysentery
        C:-cholera
        D:-travellers diarrhea
        Correct Answer:- Option-C

Question44:-Inverted fir tree appearing growth pattern in gelatin stabb culture is related to
        A:-B. anthracis
        B:-Y.pestis
        C:-P.aeruginosa
        D:-B.cereus
        Correct Answer:- Option-A

Question45:-Streptobacillus monoliformis causes
        A:-Haverhill fever
        B:-Pneumonia
        C:-Meningitis
        D:-Brazilian Purpuric fever
        Correct Answer:- Option-A

Question46:-Example of motile Gram positive bacillus is
        A:-Anthrax bacillus
        B:-Cl. perfringens
        C:-Cl. tetani
        D:-Cl.Septicum
        Correct Answer:- Option-D

Question47:-Friedlanders bacillus is
        A:-K. pneumoniae
        B:-M. tuberculosis
        C:-S.typhi
        D:-P.aeruginosa
        Correct Answer:- Option-A

Question48:-Example of transport media is
        A:-Mc Leod's medium
        B:-DCA
        C:-Stuart's medium
        D:-Mac Conkeys medium
        Correct Answer:- Option-C

Question49:-"Reynolds - Braude" phenomenon is related to
        A:-C.tropicalis
        B:-C.krusei
        C:-C.pseudotropicalis
        D:-C.albicans
        Correct Answer:- Option-D

Question50:-Aflatoxin is produced by
        A:-Rhzopus
        B:-Aspergillus fumigatus
        C:-Aspergillus flavus
        D:-Histoplasma capsulatum
        Correct Answer:- Option-C

Question51:-South American Blasto mycosis is
        A:-Histoplasmosis
        B:-Paracoccidioidomycosis
        C:-Cryptococcosis
        D:-Coccidioidomycosis
        Correct Answer:- Option-B

Question52:-Ring worm of the foot is
        A:-Tinea corporis
        B:-Tinea cruris
        C:-Tinea capitis
        D:-Tinea pedis
        Correct Answer:- Option-D

Question53:-Torulosis is caused by
        A:-C.neoformans
        B:-B.dermatitidis
        C:-P.brasiliensis
        D:-C.immitis
        Correct Answer:- Option-A

Question54:-Sabouraud's Dextrose agar does not contain
        A:-dextrose
        B:-peptone
        C:-agar
        D:- yeast extract
        Correct Answer:- Option-D

Question55:-Causative agent of "Shipyard eye" is
        A:-Herpes virus
        B:-Echo virus
        C:-Adeno virus
        D:-Arbovirus
        Correct Answer:- Option-C

Question56:-Infectious Hepatitis is
        A:-Type B Hepatitis
        B:-Type A Hepatitis
        C:-Type C Hepatitis
        D:-Type D Hepatitis
        Correct Answer:- Option-B

Question57:-HIV belongs to the family
        A:-Herpesvirus
        B:-Flavivirus
        C:-Parvovirus
        D:-Retrovirus
        Correct Answer:- Option-D

Question58:-Example of DNA virus is
        A:-Adenovirus
        B:-Retrovirus
        C:-Corona virus
        D:-Picornavirus
        Correct Answer:- Option-A

Question59:-Inclusion bodies seen in case of "fowl pox" are
        A:-Lipschutz bodies
        B:-Guarnieri bodies
        C:-Molluscum bodies
        D:-Bollinger bodies
        Correct Answer:- Option-D

Question60:-Tzanck cells seen in microscopic examination of the lesions in case of infection with
        A:-Pox virus
        B:-Herpes virus
        C:-Mumps virus
        D:-Togavirus
        Correct Answer:- Option-B

Question61:-The main component of glass is
        A:-Silicon trioxide
        B:-Silicon dioxide
        C:-Sodium dioxide
        D:-Calcium silicate
        Correct Answer:- Option-B

Question62:-Which acid is used in the preparation of chromic acid
        A:-Hcl
        B:-H₂SO₄
        C:-HNO₃
        D:-CH₃COOH
        Correct Answer:- Option-B

Question63:-Needle prick injury belongs to
        A:-Physical hazard
        B:-Chemical hazard
        C:-Biological hazard
        D:-Electric hazard
        Correct Answer:- Option-C

Question64:-Pririe acid is stored
        A:-in closed cabinet
        B:-Under water
        C:-crack proof containers
        D:-at ground level
        Correct Answer:- Option-B

Question65:-Still is used for the preparation of
        A:-Reagents
        B:-Distilled water
        C:-Acids
        D:-All of the above
        Correct Answer:- Option-B

Question66:-Unit of Luminous intensity is
        A:-Ampere
        B:-Candela
        C:-Kelvin
        D:-Mole
        Correct Answer:- Option-B

Question67:-Gram equivalent weight of a solute in a litre of solution is termed as
        A:-Molar solution
        B:-Normal solution
        C:-Percent solution
        D:-Molal solution
        Correct Answer:- Option-A

Question68:-Suitable antioagulant for blood gas analysis is
        A:-EDTA
        B:-Sodium citrate
        C:-Heparin
        D:-Double oxalate
        Correct Answer:- Option-C

Question69:-Gray coloured vacutainer tube stopper indicate the anticoagulant
        A:-EDTA
        B:-Heparin
        C:-Sodium fluoride-potassium oxalate
        D:-Citrate
        Correct Answer:- Option-C

Question70:-Chemical wastes are collected in which colour coded bag before disposal
        A:-Black
        B:-Blue
        C:-Red
        D:-Yellow
        Correct Answer:- Option-A

Question71:-For determination of electrolyte concentration, the instrument used is
        A:-fluorimeter
        B:-flame photometer
        C:-spectrophotometer
        D:-Luminometer
        Correct Answer:- Option-B

Question72:-Which of the following radioactive isotope is used to measure the activity of tyroid gland?
        A:-Cobalt-60
        B:-Carbon-14
        C:-Iodine-131
        D:-Sodium-24
        Correct Answer:- Option-C

Question73:-Henderson-Hasselbach equation is
        A:-pH=pKa+log[base]/[acid]
        B:-pH=pKa-log[base]/[acid]
        C:-pH=pKa(log[base]/[acid])
        D:-None of these
        Correct Answer:- Option-A

Question74:- pH of plasma is
        A:-6.8 - 8.4
        B:-7.3 - 7.4
        C:-5.8 - 6.2
        D:-7.1 - 7.9
        Correct Answer:- Option-B

Question75:-In thin layer chromatography, the stationary phase is
        A:-Paper
        B:-Cellulose
        C:-Resins
        D:-Gas
        Correct Answer:- Option-B

Question76:-The electrostatic interaction between charged proteins and matrix is involved in
        A:-Thin layer chromatography
        B:-Ion exchange chromatography
        C:-Molecular sieve chromatography
        D:-None of these
        Correct Answer:- Option-B

Question77:-In dry chemistry analysers, the reaction is measured by
        A:-Flame photometry
        B:-Colosemetry
        C:-Reflectance spectrophotometry
        D:-All of the above
        Correct Answer:- Option-C

Question78:-Closeness to the true value is
        A:-Accuracy
        B:-Precision
        C:-Sensitivity
        D:-None of these
        Correct Answer:- Option-A

Question79:-In accurate pepetting, belongs to which of the following?
        A:-Pre analytical error
        B:-Analytical error
        C:-Post analytical error
        D:-All the above
        Correct Answer:- Option-B

Question80:-Red coloured vacutainer tube contain,
        A:-EDTA
        B:-No any anticoagulant
        C:-Heparin
        D:-Double oxalate
        Correct Answer:- Option-B

Question81:-Estimation of serum level of which of the following parameters helps in differentiation between salivary gland lesions and pancreatic disease?
        A:-Alkaline phosphatase
        B:-Amylase
        C:-C-Reactive protein
        D:-Lipase
        Correct Answer:- Option-D

Question82:-Using Friedewald equation the LDL cholesterol value can be calculated if we know the values of
        A:-Total cholesterol and HDL cholesterol
        B:-Total cholesterol and VLDL cholesterol
        C:-Total cholesterol, HDL cholesterol and Triglycerides
        D:- Total cholesterol, HDL cholesterol and VLDL cholesterol
        Correct Answer:- Option-C

Question83:-Estimation of serum concentration of 25-hydroxy vitamin D is useful in cases of
        A:-Megaloblastic anemia
        B:-Night blindness
        C:-Osteomalacia
        D:-Pernicious anemia
        Correct Answer:- Option-C

Question84:-Urinary ALA (delta amino levulinic acid) excretion is normal in which of the following disorders
        A:-Acute intermittent porphyria
        B:-ALA dehydratase deficiency
        C:-Lead poisoning
        D:-Variegate porphyria
        Correct Answer:- Option-D

Question85:-The Michaelis constant is numerically equal to the substrate concentration when the velocity of the reaction catalysed is equal to
        A:-Vmax
        B:-Vmax/2
        C:-2 × Vmax
        D:-Zero
        Correct Answer:- Option-B

Question86:-Which of the following is true regarding kinetic assays in comparison to end point assays
        A:-Good accuracy and precision
        B:-Can be done on ordinary photometers
        C:-Cheaper
        D:-More time required
        Correct Answer:- Option-A

Question87:-Creatine phospho kinase MB isoenzyme is assayed for diagnosis of disorder of the
        A:-Brain
        B:-Heart
        C:-Liver
        D:-Kidney
        Correct Answer:- Option-B

Question88:-Thyroid stimulating hormone level estimation is commonly performed by
        A:-End point assay
        B:-Immuno assay
        C:-Kinetic assay
        D:-Potentiometry
        Correct Answer:- Option-B

Question89:-In eukaryotes the initiator codon codes for which of the following amino acids?
        A:-Methionine
        B:-Threonine
        C:-Tryptophan
        D:-Valine
        Correct Answer:- Option-A

Question90:-The substance identified by using RTPCR is
        A:-Antibody
        B:-Antigen
        C:-DNA
        D:-RNA
        Correct Answer:- Option-D

Question91:-The enzyme used for creation of a gene library is
        A:-Restriction endonuclease
        B:-Reverse transcriptase
        C:-Telomerase
        D:-Topoisomerase
        Correct Answer:- Option-A

Question92:-Adequacy of collection of a 24 hour urine specimen is analysed by noting the value of
        A:-Albumin
        B:-Creatinine
        C:-Urea
        D:-Uric acid
        Correct Answer:- Option-B

Question93:-Interference of red blood cells in CSF protein analysis is avoided by
        A:-Centrifugation
        B:-Dilution 1:10
        C:-Filtration
        D:-Precipitation
        Correct Answer:- Option-A

Question94:-The indicator used for determination of free acidity in gastric juice is
        A:-Methyl orange
        B:-Methyl red
        C:-Phenolphthalein
        D:-Topfer's reagent
        Correct Answer:- Option-D

Question95:-The most common types of kidney stones are
        A:-Calcium oxalate
        B:-Cystine
        C:-Struvite
        D:-Uric acid
        Correct Answer:- Option-A

Question96:-In serum protein electrophoresis, the globulin fraction with the slowest
rate of migration is
        A:-Alpha 1
        B:-Alpha 2
        C:-Beta
        D:-Gamma
        Correct Answer:- Option-D

Question97:-The immunoglobulin class important in secondary immune response is
        A:-IgA
        B:-IgD
        C:-IgG
        D:-IgM
        Correct Answer:- Option-C

Question98:-The enzyme present in enzyme-labelled antibodies used in ELISA technique is
        A:-5-nucleotidase
        B:-Alanine aminotransferase
        C:-Alkaline phosphatase
        D:-Aspartate aminotransferase
        Correct Answer:- Option-C

Question99:-The best index of long-term (6-8 weeks) control of blood glucose level is
        A:-Fasting Plasma Glucose
        B:-Fructosamine
        C:-Glycated Hemoglobin
        D:-Urine Microalbumin
        Correct Answer:- Option-C

Question100:-The common specimens used for drug abuse testing in employees are all of the following except
        A:-Hair
        B:-Nail
        C:-Saliva
        D:-Urine
        Correct Answer:- Option-B